Degradation of Quinoline and Indole by Thermophilic Bacteria (Anoxybacillusrupiensis Ir2 (JQ912240))

Anoxybacillus rupiensis Ir2 (JQ912240), thermophilic bacteria capable of utilizing aromatic hydrocarbon especially N-compounds that form part of petroleum components.In an attempt to investigate the ability of this bacterium to degrade quinoline and indole, the strain Ir2 was grown on these N-compounds separately as a sole source of carbon and nitrogen. Results showed that strain Ir2 was able to degrade these compounds.Optimum conditions for degradation of quinoline and indole by strain Ir2 was investigated. It was found that these conditions are growing this bacterium in mineral salt medium (pH 7 for both quinoline and indole) containing (60mM) of quinoline and (90mM) of indole, and incubated with shaking (150rpm) at 55 °C for seven days. To confirm the ability of A.rupiensis Ir2 (JQ912240) to utilize the aromatic compounds(quinoline and indole), analytical experiments HPLC was used. Results indicated that thisbacterium showed as much as 99.55% consumption of quinoline and 96.11% of indole.PCR amplification with narG2Respiratory nitrate reductase primer reveals the presence of that system in this bacterium.